In defense of these women, they were probably just confused as to how you wished to be addressed. You didn't describe how you were presenting at the time, but not everyone that presents androgynously wishes to be called "ma'am", and your description doesn't make it sound like they were deliberately being rude.
It really is a "G" concern, not a "GG" concern. They generally don't test you for a Y chromosome before behaving this way, so plenty of trans females get similar treatment. I have to walk several blocks downtown from the mass transit line to the university almost every day. It's a very well patroled area, with significant police presence most of the time, but it's not always pleasant (and I simply won't walk some places without an escort, especially after dark).