I thought I'd toss out a few of the things that produced negative reactions or even punishment when I was growing up - those that resulted in inculcating and enforcing the male role and ultimately in suppression of TG identity and expression. Just a sample, and I'd be interested in what you would add. Reactions could come from anyone, not just family. I can think of specific, swift corrections in many cases.
Emotion generally
Facial expression
Crying
Laughing
Singing
Dancing
Walk
Hip motion
Use/positioning of Hands
Moving fingers while walking
Clothing color
Clothing selection
Personal space
Touching, same sex friends
Touching, opposite sex friends
Friendships with opposite sex
Avocations and hobbies generally
Involvement with music
Interest in art
Toy choices
Book choices
Movie choices
Playing dress-Up
Playing tea
Playing with dolls
Watching girls/women's TV
Rejecting interest in football & baseball
Bedroom decorating choices/desires
Spending time with neighborhood moms
Lea