I think mannerisms come with the territory; some due to body structure and others acquired through the clothes you wear. Once at my local mall there were a group of young teenage girls who were wearing very high heels, and one was stumbling all over the place. They were having a lot of fun, but it was obvious they had never ever worn a super high heels. Maybe they were practicing for a high school prom. It was hysterically funny to watch.

Anyway, learning to NOT walk like a man is high on the list; Don't take long strides, throwing your big feel out there. Plenty of instructional Youtube videos for that.

Constantly, pushing that stray hair out of my face when I have my longish wig on.
Smoothing the back of my dress when I sit down.
Keeping my knees together when sitting.
Crossing my legs, knee over knee rather than stretched out at the ankles.
When getting into my car in a dress, sitting down facing out, first, then swiveling in, rather than sticking my right legs in first.
Bending at the knees when picking something off the floor and making sure the heel of my shoe does not step on the hem of my dress.

Adjusting a strap of a bra or slip without consciously thinking about it. Once I saw a GG reach under her dress and pull up her falling down thigh highs without missing a beat in her conversation with a friend; right in front of a crowd in a grocery store. To me, that was the ultimate in subconscious womanly mannerisms/behavior.

If I can think of others, I'll amend later.